Tara Liyanage

Biography:

Tara is a graduate student expected to graduate in the Fall of 2020. She is currently working on developing in-source HDX methodologies for characterization of carbohydrate structures with the ultimate goal of characterizing solvated glycan conformations.

Current Area:

Fundamentals of Electrospray Ionization Mass Spectrometry

Hydrogen/Deuterium Exchange is a very robust technique that is widely used to study protein structure and dynamics. However, the application of HDX-MS methodology to study carbohydrate structure has not shown the same usefulness as it has shown for proteins due to several limitations. My project focuses on addressing the current limitations of this technique to make HDX-MS methodologies applicable to study carbohydrate structure and dynamics.

Presentations:

Comparative study of in-ESI, solution-phase, and gas-phase hydrogen/deuterium exchange for conformational analysis of carbohydrate-metal adducts. session presented at: 65th Annual American Society for Mass Spectrometry Conference, (June 2017) Indianapolis, IN.

Characterizing the Forward- and Backward- Hydrogen/Deuterium Exchange of Hydroxyls Resulting from Residual Solvent Vapors in Electrospray Sources. session presented at: 65th Annual American Society for Mass Spectrometry Conference, (June 2017) Indianapolis, IN.

Applying Internal Standards to In-Electrospray – Hydrogen/Deuterium Exchange – Mass Spectrometry to Differentiate Carbohydrate Isomers. session presented at: 66th Annual American Society for Mass Spectrometry Conference, (June 2018) San Diego, CA.

Comparing Hydrogen/Deuterium Exchange – Mass Spectrometry Methods for Sampling Solvated Carbohydrate Conformations. session presented at: 66th Annual American Society for Mass Spectrometry Conference, (June 2018) San Diego, CA.

Publications:

1. Kim, H. J.; Liyanage, O. T.; Mulenos, M. R.; Gallagher, E. S. Mass Spectral Detection of Forward- and Reverse-Hydrogen/Deuterium Exchange Resulting from Residual Solvent Vapors in Electrospray Sources. J Am Soc Mass Spectrom. 2018, 29 (10), 2030-2040. DOI: 10.1007/s13361-018-2019-6

2. Liyanage, O. T.; Brantley, M. R.; Calixte, E. I.; Solouki, T.; Shuford, K. L.; Gallagher, E. S. Characterization of Electrospray Ionization (ESI) Parameters on In-ESI Hydrogen/Deuterium Exchange of Carbohydrate-Metal Ion Adducts. J. Am. Soc. Mass Spectrom. 2019, 30 (2), 235–247. DOI: 10.1007/s13361-018-2080-1

3. J. Baker, K. H. M. Al Furaiji, O. T. Liyanage, D. J. D. Wilson, J. L. Dutton, C. D. Martin, Diverse Reactivity of Dienes with Pentaphenylborole and 1‐Phenyl‐2,3,4,5‐Tetramethylborole Dimer. Chem. Eur. J. 2019, 25, 1581.

4. Liyanage, O. T.; Seneviratne, C. A.; Gallagher, E. S. Applying an Internal Standard to Improve the Repeatability of In-Electrospray H/D Exchange of Carbohydrate-Metal Adducts. J. Am. Soc. Mass Spectrom. 2019, 30, 1368-1372. DOI: 10.1007/s13361-019-02153-2

5. Hatvany, J. B.; Liyanage, O. T.; Gallagher, E. S. Effect of pH on In-Electrospray Hydrogen/Deuterium Exchange of Carbohydrates and Peptides. J. Am. Soc. Mass Spectrom. 2024, 35 (3), 441-448. DOI: 10.1021/jasms.3c00341

6. Calixte, E. I.; Liyanage, O. T.; Gass, D. T.; Gallagher, E. S. Formation of Carbohydrate-Metal Adducts from Solvent Mixtures during Electrospray: A Molecular Dynamics and ESI-MS Study. J. Am. Soc. Mass Spectrom. 2021, 32 (12), 2738-2745. DOI: 10.1021/jasms.1c00179

7. Liyanage, O. T.; Quintero, A. V.; Hatvany, J. B.; Gallagher, E. S. Distinguishing Carbohydrate Isomers with Rapid Hydrogen/Deuterium Exchange-Mass Spectrometry. J. Am. Soc. Mass. Spectrom. 2020, 32 (1), 152-156. DOI: 10.1021/jasms.0c00314

8. Calixte, E. I.; Liyanage, O. T.; Kim, H. J.; Ziperman, E. D.; Pearson, A. J.; Gallagher, E. S. Release of Carbohydrate-Metal Adducts from Electrospray Droplets: Insight into Glycan Ionization by Electrospray. J. Phys. Chem. B. 2019, 124 (3), 479-486. DOI: 10.1021/acs.jpcb.9b10369

9. Quintero, A. V.; Liyanage, O. T., Kim, H. J.; Gallagher, E. S. Characterizing the Dynamics of Solvated Disaccharides with In-Electrospray Ionization Hydrogen/Deuterium Exchange-Mass Spectrometry. Anal. Chem. 2024, 96 (31), 12649-12657. DOI: 10.1021/acs.analchem.4c01136